My all-time favorite:
"You're gonna need a bigger boat." - Martin Brody, Roy Scheider, Jaws
My next eleven all-time favorites:
"May the Force be with you." - Han Solo, Harrison Ford, Star Wars Episode IV: A New Hope
"Get away from her, you [expletive]!" - Ripley, Sigourney Weaver, Aliens
"Go ahead, make my day" - Harry Callahan, Clint Eastwood, Sudden Impact
"I'm as mad as hell, and I'm not going to take this anymore!" - Howard Beale, Peter Finch, Network
"If there is one more plague on Egypt, it is by your word that God will bring it. And there shall be so great a cry throughout the land, that you will surely let the people go." - Moses, Charlton Heston, The Ten Commandments
"We didn't land on Plymouth Rock. Plymouth Rock landed on us! " - Malcolm X, Denzel Washington, Malcolm X
"I'll be back." -The Terminator, Arnold Schwarzenegger, The Terminator
"Frankly, my dear, I don't give a damn." - Rhett Butler, Clark Gable, Gone with the Wind
"Made it, Ma! Top of the world!" - Arthur Cody Jarrett, James Cagney, White Heat
"Get your stinking paws off me, you damned dirty ape!" - George Taylor, Charlton Heston, Planet of the Apes
"Rosebud." - Charles Foster Kane, Orson Welles, Citizen Kane